Significant Treatment Effect Research Articles

PSIII-18 Effects of HiPhorius and Ronozyme HiPhos on performance of nursery pigs

Abstract The objective of this study was to evaluate the efficacy of the new phytase HiPhorius (encoded by a 6-phytase gene from Citrobacter braakii and expressed from Aspergillus oryzae; dsm-firmenich) compared with Ronozyme HiPhos (a 6-phytase product produced with a genetically modified strain of the filamentous fungus Aspergillus oryzae; dsm-firmenich) on performance of nursery pigs. A total of 528 weaned pigs [PIC Camborough female × PIC 337 boar; 19 d of age, initial body weight (BW) = 6.5 kg] were blocked by initial BW and gender and allotted to 48 pens which were randomly assigned to 1 of 4 dietary treatments (10 to 12 pigs/pen; 12 pens/treatment), including T1) Ronozyme HiPhos 3,000 FYT/kg, T2) HiPhorius 1,000 FYT/kg, T3) HiPhorius 1,500 FYT/kg, and T4) HiPhorius 2,000 FYT/kg. Pigs were fed a 3-phase feed-budget program, consisting of 1.4 kg/pig in Phase 1, 5.5 kg/pig in Phase 2, and Phase 3 diets fed to d 42 postweaning. Diets were formulated to be isocaloric and contained 1.41, 1.34, and 1.27% SID Lys for phase 1, 2, and 3, respectively. Phytase levels were tested at the same dose in phase 1 and 2 diets, while in phase 3, phytase levels were tested at 2,000 and 1,000 FYT/kg, respectively, for the Ronozyme HiPhos and HiPhorius treatments. During d 0-15 and d 0-22, pigs fed HiPhorius 1000 had reduced (P < 0.05) feed conversion than Ronozyme HiPhos and HiPhorius 2000. No significant treatment effects were observed on average daily gain (ADG) or average daily feed intake (ADFI) during these periods. During d 22-42, pigs fed HiPhorius 2,000 had greater ADG (P = 0.08), final BW (P = 0.09), and ADFI (P = 0.03) than HiPhorius 1,000 and Ronozyme HiPhos; however, there were no treatment effects on feed conversion. Overall (d 0 - 42), feeding HiPhorius 2,000 improved ADG compared with feeding Ronozyme HiPhos, HiPhorius 1,000, and HiPhorius 1,500 (476 vs. 458, 449, and 458 g, respectively; P = 0.07); however, similar ADFI and feed conversion were observed among treatments. Data from this study suggest that feeding HiPhorius at 2,000 FYT/kg provided greater ADG compared with feeding HiPhorius at reduced amounts or Ronozyme HiPhos. While the two sources of phytase had similar responses on nursery ADFI and feed conversion overall, feeding HiPhorius at 1,000 FYT/kg resulted in decreased feed conversion during the first 3 wk post-weaning.

260 Effect of different doses of phytase (HiPhorius) and two primary calcium sources on nutrient digestibility, retention and blood parameters in weaned piglets

Abstract An 18-d study evaluated the effect of increasing phytase levels in diets with limestone or Ca-formate on Ca and P digestibility, P retention, and blood inositol, Ca, and P in weaned piglets. At weaning, male piglets [n = 60; 29.8 ± 1.10 d of age; initial body weight (BW) = 8.8 ± 0.31 kg) were randomly allocated to ten diets formulated as a 2 × 5 factorial design with 2 primary Ca sources (limestone or Ca-formate) and 5 Ca/P levels. There was a positive control diet (PC; 0.76% Ca, 0.34 dP) and 4 negative control diets (NC; 0.56% Ca, 0.34% dP) supplemented with phytase at 0, 750, 1,500, and 3,000 FYT/kg. At d 10, piglets were individually housed in metabolism crates and fed 3.2 x metabolic weight. On d 14 to 18, fecal and total urine samples were collected. Apparent total tract digestibility (ATTD) was calculated for dry matter (DM), organic matter (OM), crude protein (CP), ash, Ca, and P using TiO2 as marker. Urinary P was measured to calculate P retention. These data were analyzed as a 2-way interaction. On d 0 (baseline), 8 and 18, blood samples were collected to analyze serum Ca and P, and plasma inositol. Blood data were analyzed as a 3-way interaction. Results indicated that piglets fed Ca-formate had greater ATTD of Ca (75.0% vs. 71.4%, P < 0.01) but decreased ATTD of P (66.4% vs. 68.8%, P < 0.01), than piglets fed limestone. Phytase supplementation improved ATTD of DM, ash, OM, CP, P, and Ca compared with the NC and PC. An interaction between Ca/P levels and Ca-sources was observed for urinary P (P = 0.03), as there were no differences between Ca sources in piglets fed the NC or the 750 FYT diets, but piglets fed the PC diet and the NC+1,500 or 3,000 FYT with limestone had greater urinary P compared with Ca-formate. A 2-way interaction between Ca/P levels and d was observed for plasma inositol (P = 0.012). On d 8 and 18, there was no difference between the NC and PC;, however, phytase supplementation at 750, 1,500, and 3,000 FYT increased plasma inositol on d 8 (58.2, 80.7, and 89.5 μmol) and d 18 (70.2, 85.4, and 106.4 μmol). There was only a main effect of Ca/P levels in serum P. Compared with the NC (73.7 mg/L), the PC and the NC+750, 1,500, and 3,000 FTU/kg increased serum P (90.3, 89.1, 87.6, and 93.2 mg/L, respectively, P < 0.01). There was no effect of Ca source on blood inositol and P. No significant dietary treatment effects were observed in serum Ca. In conclusion, using Ca-formate instead of limestone increases Ca digestibility but reduces P digestibility, likely due to a greater Ca solubility of Ca-formate. This suggests that diets should be formulated based on digestible Ca instead of total Ca.

102 Circadian patterns of behavior, respiration, rectal temperature, saliva melatonin, cytokine concentrations, and milk immune cells are altered by cooling heat-stressed lactating sows

Abstract Lactating sows are particularly vulnerable to heat stress (HS) due to the increased metabolism from milk production. A greater understanding of HS response could assist in mitigating its detrimental effects on animal production. Two core circadian clock genes were pinpointed in a recent genome study as potential mediators of HS response, indicating the possible alteration of circadian clocks by HS exposure. This study aimed to assess how cooling of lactating sows under natural heat stress conditions in the summer influenced circadian patterns of respiration rate (RR), rectal temperature (RT), behaviors, salivary concentration of cortisol and melatonin, milk cell populations and relative abundance of multiple cytokines in saliva. Lactating sows of mixed parities were divided into two treatment groups: treated with electronic cooling pad (C; n=9) and heat-stressed control (H; n=9). Experiments were conducted during two 48 h periods of increased environmental temperatures from summer heatwaves. During the initial 48 h period, rectal temperature (RT) was monitored every 60 min, rectal temperature (RT) every 30 min, and behaviors of eating, standing, sitting, laying, sleeping, drinking, and nursing every 5 min. Morning milk samples were collected following the completion of the first phase and utilized to categorize and count somatic cells. The second trial was approximately 1 wk later, and during this period saliva samples were collected every 4 h over the 48-h period. Results were analyzed for significant effect of treatments and interaction using periodic regression while circadian patterns were further detected by incorporating a Fourier series model utilizing sine-cosine variables that denote a fit and/or shift in circadian pattern. The analysis revealed that cooling decreased RT and RR while also modifying circadian patterns (P < 0.05). Cooling had no impact on the percentage of time spent in any activity over the 48-h period (P > 0.05). Nevertheless, the daily patterns of eating, standing, and sitting were different between the treatments (P < 0.05). A shift in eating time to later in the day in H sows relative to C sows related to a second rise in RT during the dark phase of the light-dark cycle (P < 0.05). Saliva melatonin concentrations were increased in C sows and circadian pattern altered relative to H (P < 0.05), but there was no effect on salivary cortisol concentrations (P > 0.05). Cooling reduced milk lymphocytes (P < 0.05) and influenced diurnal pattern of saliva cytokines including interleukins (IL) IL-1 alpha, IL-1 beta, IL-10, and IL-15 and transforming growth factor-beta 1. In conclusion, the cooling of heat-stressed sows altered the circadian patterns of physiology, behavior and melatonin, suggesting the need for additional research to investigate whether circadian disruption contributes to reduced production efficiency observed in HS animals.

Learning optimal biomarker-guided treatment policy for chronic disorders.

Electroencephalogram (EEG) provides noninvasive measures of brain activity and is found to be valuable for the diagnosis of some chronic disorders. Specifically, pre-treatment EEG signals in the alpha and theta frequency bands have demonstrated some association with antidepressant response, which is well-known to have a low response rate. We aim to design an integrated pipeline that improves the response rate of patients with major depressive disorder by developing a treatment policy guided by the resting state pre-treatment EEG recordings and other treatment effects modifiers. First, we design an innovative automatic site-specific EEG preprocessing pipeline to extract features with stronger signals than raw data. We then estimate the conditional average treatment effect (CATE) using causal forests and use a doubly robust technique to improve efficiency in the estimation of the average treatment effect. We present evidence of heterogeneity in the treatment effect and the modifying power of the EEG features, as well as a significant average treatment effect, a result that cannot be obtained with conventional methods. Finally, we employ an efficient policy learning algorithm to learn an optimal depth-2 treatment assignment decision tree and compare its performance with Q-Learning and outcome-weighted learning via simulation studies and an application to a large multi-site, double-blind, randomized controlled clinical trial, EMBARC.

Innovative decision making tools using discrete mathematics for stress urinary incontinence treatment.

In this study, we applied graph theory to clinical decision-making for Stress Urinary Incontinence (SUI) treatment. Utilizing discrete mathematics, we developed a system to visually understand the shortest path to the desired treatment outcomes by considering various patient variables. Focusing on women aged 35-50, we examined the effectiveness of Tension-free Vaginal Tape (TVT) surgery and Vaginal Erbium Laser (VEL) treatment for over 15years. The TVT group consisted of 102 patients who underwent surgery using either the Advantage Fit mid-urethral sling system (Boston Scientific Co., MA, USA) or the GYNECARE TVT retropubic system (Ethicon Inc., NJ, USA). The VEL group included 113 patients treated with a non-ablative Erbium: YAG laser (FotonaSmooth™ XS; Fotona d.o.o., Ljubljana, Slovenia), and there were 112 patients in the control group. We constructed a network diagram analyzing the correlations between health, demographic factors, treatment methods, and patient outcomes. By calculating the shortest path using heuristic functions, we identified significant correlations and treatment effects. This approach supports patient decision making by choosing between TVT and VEL treatments based on individual objectives. Our findings provide new insights into SUI treatment, highlighting the value of a data-driven personalized approach for clinical decision-making. This interdisciplinary study bridges the gap between mathematics and medicine, demonstrating the importance of a data-centric approach in clinical decisions.

The Effect of a Mental Health Intervention on Health Behaviors among Cancer Survivors

The Effect of a Mental Health Intervention on Health Behaviors among Cancer Survivors

The addition of bortezomib to rituximab, high-dose cytarabine and dexamethasone in relapsed or refractory mantle cell lymphoma-a randomized, open-label phase III trial of the European mantle cell lymphoma network.

The therapy of relapsed or refractory (r/r) mantle cell lymphoma (MCL) patients remains a major clinical challenge to date. We conducted a randomized, open-label, parallel-group phase-III trial hypothesizing superior efficacy of rituximab, high-dose cytarabine and dexamethasone with bortezomib (R-HAD + B) versus without (R-HAD) in r/r MCL ineligible for or relapsed after autologous stem cell transplant (ASCT). Primary endpoint was time to treatment failure (TTF), secondary endpoints included response rates, progression free survival, overall survival, and safety. In total, 128 of 175 planned patients were randomized to R-HAD + B (n = 64) or R-HAD (n = 64). Median TTF was 12 vs. 2.6 months (p = 0.045, MIPI-adjusted HR 0.69; 95%CI 0.47-1.02). Overall and complete response rates were 63 vs. 45% (p = 0.049) and 42 vs. 19% (p = 0.0062). A significant treatment effect was seen in the subgroup of patients >65 years (aHR 0.48, 0.29-0.79) and without previous ASCT (aHR 0.52, 0.28-0.96). Toxicity was mostly hematological and attributable to the chemotherapeutic backbone. Grade ≥3 leukocytopenia and lymphocytopenia were more common in R-HAD + B without differences in severe infections between both arms. Bortezomib in combination with chemotherapy can be effective in r/r MCL and should be evaluated further as a therapeutic option, especially if therapy with BTK inhibitors is not an option. Trial registration: NCT01449344.

Summer cover crop and temporary legume-cereal intercrop effects on soil microbial indicators, soil water and cash crop yields in a semi-arid environment

ContextFew studies have investigated summer cover crops as a replacement for bare fallow in semi-arid temperate regions, and summer cover crop impacts on soil biological function, water balances and subsequent winter crop yields in these regions remain largely unknown. ObjectiveThe 4-year field study aimed to determine the consequences of replacing a summer fallow with a summer growing cover crop on soil function, water balances and subsequent winter cash crop yields in a semi-arid region (486 mm mean annual rainfall) of the Australian southern cropping zone. MethodsA canola (Brassica napus)-wheat (Triticum aestivum L.) rotation with summer fallow was compared to the same rotation with either i) a single species (buckwheat, Fagopyrum esculentum L.) summer cover crop; ii) a four-species mixed brassica + cereal summer cover crop or iii) a cropping sequence with a temporary legume intercrop in the wheat phase of the rotation. Cover crop biomass production, soil water to a depth of 90 cm at cash crop sowing, crop yields and soil functional indices were quantified. ResultsCover crops produced 500–700 kg biomass ha−1 in the 2020 and 2021 seasons and 1000–2400 kg biomass ha−1 in 2022. The four-species mixed cover crop significantly lowered soil water (around 30 mm; P < 0.05) at the time of sowing the cash crop compared to the control and resulted in 15% reduction (P = 0.12) in canola seed yield in 2020, but no significant effect of cover crops of soil water and mineral N at sowing, or cash crop yields, were observed in subsequent seasons. In 2021 the temporary vetch intercrop produced > 1000 kg ha−1 biomass over 9 weeks, and led to a (non-significant) 10% wheat yield reduction. There was no significant effect of cover crop or temporary intercrop treatments on soil functions in the 2020 or 2021 seasons, but activities of several soil enzymes (leucine aminopeptidase, arylsulfatase and B-glucosidase; P < 0.05) and water-soluble carbon (P = 0.059) were significantly higher in the summer cover crop plots compared to control and intercrop plots in March 2022 following termination of the cover crops. These changes coincided with a significant increase in saturated hydraulic conductivity in the four-species cover crop plots in March 2022 compared to control plots. ImplicationsThe study did not provide any compelling evidence for benefits of summer cover crops over fallow in the short term (3–4 years). The relatively low cover crop biomass production over the 4-year study suggests summer cover crops are unlikely to substantially improve soil carbon levels in temperate, semi-arid cropping systems.

Efficacy of CEITA early intervention program for young children with autism spectrum disorders: A randomized controlled trial design

Background This study examined the efficacy of the Comprehensive Early Intervention for Toddlers with Autism (CEITA) program for children with autism spectrum disorders in Malaysia. Methods A randomized control trial design was adopted, in which 16 children (mean age= 34.25 months) were assigned to either the intervention group (n=8) or the control group (n=8). Children in the intervention group underwent three months of low intensity (2hrs/week) CEITA program intervention, whereas the control group received no treatment. Autism symptoms and developmental growth scores were measured at baseline, post-treatment, and 6-week follow-up to evaluate the efficacy of the CEITA program. Results Multivariate analysis of covariance results showed a significant intervention effect, whereby autism symptoms were significantly reduced (η2 = 0.36), and growth scores for all developmental domains (η2 = 0.20-0.66) were significantly increased. Conclusion Hence, this study highlights the potential of the CEITA program’s clinical implications, and relevant future research is recommended. Trial registration Reg number: NMRR ID-22-00626-8LB, dated 29th April 2022.

[Clinical observation of sinus tarsi syndrome after lateral ankle sprain].

To study the incidence rate of sinus tarsi syndrome after lateral ankle sprain and observe the clinical efficacy of sinus tarsal corticosteroid injections. From January 2021 to Janury 2022, 391 patients with lateral ankle sprain and 88 patients with sinus tarsi syndrome using corticosteroid injections (compound betamethasone 1 ml+ lidocaine hydrochloride 4 ml) were retrospectively analyzed. There were 22 males and 66 females, aged from 29 to 60 years old with an average of (41.00±7.52) years old, duration of the disease from 1 to 12 months with an average of (5.6±4.2) months. The visual analogue scale(VAS) and American Orthopedic Foot and Ankle Society(AOFAS) scores were collected before, 1 month, 3 months, 6 months, and 12 months after treatment. All 88 patients completed a 12-month follow-up. The incidence rate of sinus tarsi syndrome after lateral ankle sprain was 22.5%. One month after treatment, VAS was 1.20±0.89, AOFAS score was 88.70±7.04. Three months after treatment, VAS was 1.60±1.35, AOFAS score was 85.20±10.95. Six months after treatment, VAS 2.35±1.39, AOFAS 80.30±9.75. Twelve months after treatment, VAS was 2.80±1.51, AOFAS score was 79.1±9.94. Significant differences were found before and after treatment at all four time points of follow-up(P<0.05). The results of this study showed that the incidence rate of sinus tarsi syndrome after lateral ankle sprain was 22.5%. Corticosteroid injections were effective in the short term with a 65% recurrence rate of symptoms within 1 year. For patients with no significant long-term effect of conservative treatment, clinicians may explore alternative approaches, including options like ankle arthroscopy.

Efficacy of mobile phone intervention to increase male partner antenatal care attendance for HIV testing in Moshi municipal, Tanzania: a randomized controlled trial

BackgroundHIV partner counselling and testing in antenatal care (ANC) is a crucial strategy to raise the number of males who know their HIV status. However, in many settings like Tanzania, male involvement in antenatal care remains low, and there is a definite need for innovative strategies to increase male partner involvement. This study was designed to evaluate the efficacy of mobile phone intervention increase male partner ANC attendance for HIV testing in Moshi municipal, Tanzania.MethodsBetween April and July 2022, we enrolled pregnant women presenting to a first ANC visit at Majengo and St. Joseph reproductive health facilities without their male partners. Eligible pregnant women were randomly assigned to invitation of their male partners either via phone calls, text messages from clinic staff and verbal invites from pregnant partners (intervention arm) or verbal invites only from the pregnant partners (control arm). Neither healthcare provider nor participant were blinded. The primary outcome was the proportion of male partners who attended ANC with their pregnant partners during a follow-up period of two consecutive visits. The secondary outcome measure was HIV testing among male partners following the invitation. Participants were analyzed as originally assigned (intention to treat).ResultsA total of 350 pregnant women presenting to ANC for the first time were enrolled, with 175 women enrolled in each arm. The efficacy of male attendance with their pregnant women following the invitations was 83.4% (147/175) in the intervention arm and 46.3% (81/175) in the control arm. Overall, the results suggest a positive and statistically significant average treatment effect among men who received mobile phone intervention on ANC attendance. For the secondary outcome, the percent of male partners who accepted HIV counselling and testing was 99.3% (146/147) in the intervention arm and 93.8% (76/81) in the control arm. Married men were having higher odds of ANC attendance compared with single men (aOR:6.40(3.26–12.56), Males with multigravida women were having lower odds of ANC attendance compared with primigravida women (aOR:0.17(0.09–0.33).ConclusionThe study demonstrates that supplementing verbal invitations with mobile phone calls and text messages from clinic staff can significantly increase male partner ANC attendance and HIV testing. This combined approach is recommended in improving ANC attendance and HIV testing of male partners who do not accompany their pregnant partners to antenatal clinics in the first visits.Trial registrationPACTR202209769991162.

Home-Based Cognitive Intervention for Healthy Older Adults Through Asking Robots Questions: Randomized Controlled Trial.

Asking questions is common in conversations, and while asking questions, we need to listen carefully to what others say and consider the perspective our questions adopt. However, difficulties persist in verifying the effect of asking questions on older adults' cognitive function due to the lack of a standardized system for conducting experiments at participants' homes. This study examined the intervention effect of cognitive training moderated by robots on healthy older adults. A focus on the feasibility of the intervention at participants' homes was also maintained. Feasibility was evaluated by considering both the dropout rate during the intervention and the number of questions posed to each participant during the experiment. We conducted a randomized controlled trial with 81 adults older than 65 years. Participants were recruited through postal invitations and then randomized into 2 groups. The intervention group (n=40) received sessions where participants listened to photo-integrated stories and posed questions to the robots. The control group (n=41) received sessions where participants listened to photo-integrated stories and only thanked the robots for confirming participation. The participants participated in 12 dialogue sessions for 2-3 weeks. Scores of global cognitive functioning tests, recall tests, and verbal fluency tasks measured before and after the intervention were compared between the 2 groups. There was no significant intervention effect on the Telephone Interview for Cognitive Status-Japanese scores, recall tests, and verbal fluency tasks. Additionally, our study successfully concluded with no participant dropouts at follow-up, confirming the feasibility of our approach. There was no statistically significant evidence indicating intervention benefits for cognitive functioning. Although the feasibility of home-based interventions was demonstrated, we identified areas for improvement in the future, such as setting up more efficient session themes. Further research is required to identify the effectiveness of an improved cognitive intervention involving the act of asking questions.

Two-year follow-up of a clustered randomised controlled trial of a multicomponent general practice intervention for people at risk of poor health outcomes

BackgroundThis study was a two-year follow-up evaluation of health service use and the cost-effectiveness of a multicomponent general practice intervention targeted at people at high risk of poor health outcomes.MethodsA two-year follow-up study of a clustered randomised controlled trial was conducted in South Australia during 2018–19, recruiting 1044 patients from three cohorts: children; adults (aged 18–64 years with two or more chronic diseases); and older adults (aged ≥ 65 years). Intervention group practices (n = 10) provided a multicomponent general practice intervention for 12 months. The intervention comprised patient enrolment to a preferred general practitioner (GP), access to longer GP appointments and timely general practice follow-up after episodes of hospital care. Health service outcomes included hospital use, specialist services and pharmaceuticals. The economic evaluation was based on quality-adjusted life years (QALYs) calculated from EuroQoL 5 dimensions, 5 level utility scores and used an A$50,000 per QALY gained threshold for determining cost-effectiveness.ResultsOver the two years, there were no statistically significant intervention effects for health service use. In the total sample, the mean total cost per patient was greater for the intervention than control group, but the number of QALYs gained in the intervention group was higher. The estimated incremental cost-effectiveness ratio (ICER) was A$18,211 per QALY gained, which is lower than the A$50,000 per QALY gained threshold used in Australia. However, the intervention's cost-effectiveness was shown to differ by cohort. For the adult cohort, the intervention was associated with higher costs and lower QALYs gained (vs the total cohort) and was not cost-effective. For the older adults cohort, the intervention was associated with lower costs (A$540 per patient), due primarily to lower hospital costs, and was more effective than usual care.ConclusionsThe positive cost-effectiveness results from the 24-month follow-up warrant replication in a study appropriately powered for outcomes such as hospital use, with an intervention period of at least two years, and targeted to older people at high risk of poor health outcomes.

Functional family therapy for adolescent disruptive behavior in Norway: Results from a randomized controlled trial.

Disruptive behavior during adolescence is linked to severe problems for the youths later in life and poses challenges to the families, schools, and treatment systems these youths meet. This randomized controlled trial was conducted to examine the short- and long-term effectiveness of functional family therapy (FFT) for adolescents aged 11-17 referred for disruptive behavior to Child Welfare Services in Norway. One hundred sixty-one youths (Mage = 14.7, 45.9% female) were randomly assigned to FFT (n = 88) or treatment as usual (TAU, n = 73). Primary outcomes were parent- and teacher-reported youth aggressive behavior, rule-breaking behavior, internalizing problems, and social skills; youth self-reported delinquency (SRD) and negative peer involvement; and teacher-reported academic performance and adaptive functioning. Outcomes were collected before treatment (pretest), 6 months after pretest (posttest), and 18 months after pretest (follow-up). The results showed no intervention effect for FFT compared to TAU between pretest and posttest (p > .05). Significant improvements between pretest and posttest were found for youth receiving both FFT and TAU on parent-reported aggressive and rule-breaking behavior, internalizing problems, and social skills (ranging from d = 0.56 to -0.45) and youth SRD (d = 0.29). Between posttest and follow-up, however, a significant intervention effect in favor of TAU was found for parent-reported youth internalizing (d = 0.27). Significant improvements between posttest and follow-up were also found for youth receiving both FFT and TAU on parent- and teacher-reported aggressive behavior. Findings did not support the hypothesized superiority of FFT over TAU. (PsycInfo Database Record (c) 2024 APA, all rights reserved).

Effects of Dietary Incorporation of Neem (Azadirachta indica), Moringa (Moringa oleifera), and Jute (Corchorus olitorius) Leaf Powder on Production Performance and Blood Indices of Broiler Chickens as a Substitute of Antibiotic

The study was planned to determine the effects of dietary incorporation of Neem (Azadirachta indica), Moringa (Moringa oleifera), and Jute (Corchorus olitorius) leaf powder on production performance, carcass traits, and blood indices of broiler chickens as a substitute for the antibiotic. A total of 200-day-old Cobb 500 broiler chicks were divided randomly into 5 experimental groups. Whereas groups T1, T2, T3, and T4 were offered basal feed supplemented with 2% Neem Leaf Powder (NLP), 2% Moringa Leaf Powder (MLP), 2% Jute Leaf Powder (JLP), and antibiotics, respectively. In this research, the total feed consumption of birds was significantly (P &lt; 0.05) lower in the T1 and T2 group than in the T3 and T4 group. Final live weight was significantly (P &lt; 0.05) higher in 2% MLP treated group T2 followed by T1, T3, and T0 group. Significantly (P &lt; 0.05) better feed conversion ratio (FCR) was found in the T2 group compared to T0, T3, and T4 groups. There found no significant (P &gt; 0.05) effect of treatments on the survivability percentage of birds. All the treatment groups showed an insignificant (P &gt; 0.05) effect on dressing percentage and organs (liver, heart, and gizzard) weight of broiler chicken. Weight of immune organs (spleen and bursa) and blood parameters (concentration of hemoglobin, glucose, and cholesterol) were not influenced (P &gt; 0.05) by dietary addition. Birds fed the lower amount of feed, higher body weight, and improved FCR were found in the 2% MLP treated group. In overall aspects, the effectiveness of 2% MLP addition was better than 2% NLP and 2% JLP as an antibiotic alternative.

FirstStep2Health: A cluster randomised trial to promote healthy behaviours and prevent obesity amongst low-income preschoolers.

To evaluate the preliminary effects of a theory-based, multi-component intervention on improving healthy lifestyle behaviours and preventing obesity amongst low-income preschoolers. A cluster randomised controlled trial was conducted at 10 daycare centres. The 16-week FirstStep2Health intervention, grounded in the Actor-Partner Interdependence Model and the Social Cognitive Theory, included five components: a Facebook-based parent programme, three virtual parent meetings, three weekly motivational text messages, parent-child learning via weekly child letters and daycare centre-based child programme. A total of 95 preschoolers (53 intervention and 42 control) participated. Preschoolers' mean age was 49.27 months, with 57.9% being female, 12.6% being Hispanic and 40% being African American. The intervention significantly decreased intervention preschoolers' fat intake (B = -33.76, p = 0.047) and % body fat (B = -1.18, p = 0.036) compared to the control. During year 2, there were significant intervention effects on increasing skin carotenoids (B = 87.06, p = 0.035). Although not statistically significant, the intervention showed positive effects on increasing preschoolers' fruit/vegetable intake; and decreasing screen time, body mass index z-score and proportion of overweight/obesity. Even with some potential limitations (small sample size, measurement concerns and confounding with Coronavirus Disease 2019 pandemic), the study's results support the preliminary efficacy of the FirstStep2Health intervention in preventing obesity amongst low-income racially diverse preschoolers.

Paradoxical effect of anti-inflammatory drugs on IL-6 mRNA expression in patients with PTSD during treatment.

The pathophysiology of posttraumatic stress disorder (PTSD) is associated with the activation of the innate immune system, including cytokines like interleukin 6 (IL-6). However, the role of IL-6 in the etiology and treatment of PTSD still remains elusive. We conducted a prospective controlled trial to investigate the development of IL-6 during psychosomatic treatment in individuals with PTSD in comparison with individuals without PTSD. We assessed IL-6 mRNA expression before and after 2 months of psychosomatic treatment in individuals with and without PTSD. Severities of PTSD and depressive symptoms were assessed in parallel. Linear mixed regression was applied for statistical analysis, including the factors diagnosis PTSD and pre-post treatment after subgrouping for intake of anti-inflammatory drugs. The development of IL-6 mRNA expression during treatment was affected by the use of anti-inflammatory drugs. In the subgroup without intake of anti-inflammatory drugs, no significant statistical treatment effect in individuals with and without PTSD emerged. In the subgroup of individuals taking anti-inflammatory drugs, a significant interaction effect of the factors pre-post treatment and diagnosis PTSD was observed. Whereas IL-6 mRNA expression in individuals without PTSD decreased according to amelioration of symptoms, IL-6 mRNA expression in individuals with PTSD increased significantly during treatment, in opposite direction to symptom severity. Anti-inflammatory drugs might affect IL-6 mRNA expression in individuals with PTSD in a paradoxical way. This study offers a further piece of evidence that IL-6 could be involved in the pathophysiology of PTSD and PTSD-specific immunologic molecular mechanisms.

Effects of a Flavonoid-Rich Blackcurrant Beverage on Markers of the Gut-Brain Axis in Healthy Females: Secondary Findings From a 4-Week Randomized Crossover Control Trial

The microbiota–gut–brain axis is a promising target to alleviate the growing burden of neurologic and mental health disorders. Dietary polyphenols act on multiple components of the microbiota–gut–brain axis, but this complex relationship requires further attention. This randomized, placebo-controlled, double-blind, crossover trial (ACTRN12622000850774) compared 4 wk a commercially available flavonoid-rich blackcurrant beverage (FBB; 151 mg anthocyanins, 308 mg total polyphenols) with placebo in 40 healthy females (18–45 y). The primary outcome of stress reactivity was assessed by change in present feelings of stress, mood, and fatigue before and after completing a 20-min cognitive stressor (Purple multitasking framework (MTF)]. Secondary end points included cognitive performance (MTF), mood [profile of mood states (POMS)], sleep (Pittsburgh Sleep Quality Index), fecal microbiome composition and functional potential (shotgun sequencing), and blood biomarker concentrations (brain-derived neurotrophic factor, tryptophan, kynurenine, and interleukin 6). Statistical analyses were conducted on an intention-to-treat basis using linear mixed-effect models. Thirty-eight participants completed both intervention arms. There was no significant treatment effect on the primary outcome of stress reactivity. Compared with placebo, working memory (letter retrieval scores and MTF), and anxiety/tension and anger/hostility domains of the POMS improved with FBB supplementation (time × intervention interaction; P < 0.05). There were no treatment effects on gut microbiome composition or functional potential. Baseline abundances of Bifidobacterium genera and species (Bifidobacterium longum and Bifidobacterium bifidum) tended to be higher in participants with the greatest improvements in letter retrieval scores with FBB supplementation (nominally significant, P < 0.05). In conclusion, 4-wk FBB supplementation improved secondary outcomes of working memory performance during multitasking and mood outcomes in healthy adult females. These results should be confirmed in a larger cohort with a longer duration of follow-up.

Raising the Stakes on a Political Science Major Field Test: Evidence From a New Capstone Course

Using a major field test (MFT) is a common method of program assessment. For accurate assessment to take place, however, students must be sufficiently motivated to perform. This paper studies a change in major requirements that associated a major field test to a grade in a political science capstone course. After controlling for possible confounders like Gender, Age, Ethnicity, and differences in GPA and ACT scores, a modest but statistically significant treatment effect was found. However, the fit between a student’s program GPA and MFT scores did not improve, leading to questions about how best to assess political science knowledge for program graduates.

Prazosin and cyproheptadine in combination in the treatment of alcohol use disorder: A randomized, double-blind, placebo-controlled trial.

Pre-clinical studies suggest that the simultaneous blockade of the α1b and 5HT2A receptors may be effective in reducing alcohol consumption. This study aimed to assess the efficacy and safety of prazosin (α1b blocker) and cyproheptadine (5HT2A blocker) combination in decreasing total alcohol consumption (TAC) in alcohol use disorder (AUD). This was a double-blind, parallel group, placebo-controlled, Phase 2, randomized clinical trial conducted in 32 addiction treatment centres in France. A total of 108 men and 46 women with severe AUD took part. Participants were randomly assigned to one of the following 3-month treatments: (1) low-dose group (LDG) receiving 8mg cyproheptadine and 5mg prazosin extended-release (ER) formulation daily; (2) high-dose group (HDG) receiving 12 mg cyproheptadine and 10mg prazosin ER daily; and (3) placebo group (PG) receiving placebo of cyproheptadine and prazosin ER. A total of 154 patients were randomized: 54 in the PG, 54 in the LDG and 46 in the HDG. The primary outcome was TAC change from baseline to month 3. A significant main treatment effect in the change in TAC was found in the intent-to-treat population (P = 0.039). The HDG and LDG showed a benefit in the change in TAC from baseline to month 3 compared with PG: -23.6g/day, P = 0.016, Cohen's d = -0.44; -18.4g/day, P = 0.048 (Bonferroni correction P < 0.025), Cohen's d = -0.36. In a subgroup of very high-risk drinking-level participants (> 100 g/day of pure alcohol for men and > 60 g/day for women), the difference between the HDG and the PG in the primary outcome was -29.8g/day (P = 0.031, Cohen's d = -0.51). The high and low doses were well-tolerated with a similar safety profile. A randomized controlled trial of treatment of severe alcohol use disorder with a cyproheptadine-prazosin combination for 3months reduced drinking by more than 23 g per day compared with placebo. A higher dose combination was associated with a larger magnitude of drinking reduction than a lower dose combination while showing similar safety profile.